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KMID : 0380219940270030224
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 3 p.224 ~ p.229
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Molecular Cloning and Sequence Analysis of Elongator tRNA Gene from Corm Mitochondria derived from Chloroplast
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Abstract
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Abstract:
@EN A genomic library was constructed by cloning large fragments of maize mtDNA into pBR322. The fragments were produced by partial digestion with HindIII. Clone H606/01 contained a 20 kb mtDNA insert consisting of two HindIII fragments of 9 kb
and
11
kb. The clone was also found to harbor an elongator methionine tRNAMet gene. This elongator tRNAMet gene is exactly identical to its chloroplast counterparts both in rice and maize, with some variation in the flanking regions. The restriction map
for
the flanking regions of the gene was compared with that of chloroplasts, and the entire 9 kb HindIII fragment insert was identical of that of cpDNA. This suggests that it is another example of a cpDNA insert present in mtDNA.
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KEYWORD
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